Do you have a protocol or proposed antibodies to conduct ICC staining on my totally differentiated ALI cultures?

After the run is comprehensive, retrieve the Shipping Cartridge through the instrument. The cell sample really should be at The underside or facet of the gathering tube. Observe: It is usually recommended to spin down the Supply Cartridge inside of a mini-centrifuge for just a few seconds for total volume Restoration.

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Alternatively, dissociate organoids using Mild Cell Dissociation Reagent (GCDR) without the need of prior retrieval of organoids in the 지방이식 Matrigel® dome. This will likely be certainly a lot quicker but bears the potential risk of insufficient cell dissociation as a result of difficulties with Matrigel® and could be a lot more stress stem cell clinic filled to the cells.

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Notes: STEMdiff™ Kidney Organoid Kit will involve a special plate structure/society set-up and isn't a standard monolayer culture; kidney organoids protrude through the fundamental monolayer culture.

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Sure, you’ll locate the step-by-phase protocol for TEER measurement To judge the epithelial barrier integrity in ALI cultures right here.

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Take away supernatant and resuspend cells 지방흡입 in FACS buffer. Note: It can be crucial to quench the dissociation reagent by using the 가슴수술 exact same or double the quantity in the dissociation reagent.